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Bromelain

Granules India Limited has high volume world-class facilities for APIs, PFIs, & FDFs, serving customers in over 80 countries.

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9 API Suppliers, 3 USDMF, 1 JDMF, 6 Listed Suppliers, $ API Reference Price

9 API Suppliers
3 USDMF
1 JDMF
6 Listed Suppliers
$ API Reference Price

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15 Drugs in Development

15 Drugs in Development

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Chemistry

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Also known as: Muxf, M0xf(3), Bromelain, technical grade, Chebi:53469, 9001-00-7, Fuc-alpha-(1->3)-[man-alpha-(1->6)-[xyl-beta-(1->2)]-man-beta-(1->4)-glcnac-beta-(1->4)]-glcnac

Molecular Formula

C₃₉H₆₆N₂O₂₉

Molecular Weight

1026.9 g/mol

InChI Key

UNMLVGNWZDHBRA-UFAVQCRNSA-N

Protein-digesting and milk-clotting enzymes found in PINEAPPLE fruit juice and stem tissue. Enzymes from the two sources are distinguished as fruit bromelain and stem bromelain. This enzyme was formerly listed as EC 3.4.22.4.

1 2D Structure

Bromelain

2 Identification

2.1 Computed Descriptors

2.1.1 IUPAC Name

N-[(2S,3R,4R,5S,6R)-2-[(2R,3S,4R,5R)-5-acetamido-6-hydroxy-2-(hydroxymethyl)-4-[(2S,3S,4R,5S,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-3-yl]oxy-5-[(2S,3S,4S,5S,6R)-4,5-dihydroxy-6-[[(2S,3S,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]-3-[(2S,3R,4S,5R)-3,4,5-trihydroxyoxan-2-yl]oxyoxan-2-yl]oxy-4-hydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide

2.1.2 InChI

InChI=1S/C39H66N2O29/c1-9-19(48)24(53)29(58)38(62-9)69-32-18(41-11(3)46)34(59)63-15(6-44)31(32)68-35-17(40-10(2)45)23(52)30(14(5-43)65-35)67-39-33(70-37-27(56)20(49)12(47)7-60-37)26(55)22(51)16(66-39)8-61-36-28(57)25(54)21(50)13(4-42)64-36/h9,12-39,42-44,47-59H,4-8H2,1-3H3,(H,40,45)(H,41,46)/t9-,12+,13+,14+,15+,16+,17+,18+,19+,20-,21+,22+,23+,24+,25-,26-,27+,28-,29-,30+,31+,32+,33-,34?,35-,36-,37-,38-,39-/m0/s1

2.1.3 InChI Key

UNMLVGNWZDHBRA-UFAVQCRNSA-N

2.1.4 Canonical SMILES

CC1C(C(C(C(O1)OC2C(C(OC(C2OC3C(C(C(C(O3)CO)OC4C(C(C(C(O4)COC5C(C(C(C(O5)CO)O)O)O)O)O)OC6C(C(C(CO6)O)O)O)O)NC(=O)C)CO)O)NC(=O)C)O)O)O

2.1.5 Isomeric SMILES

C[C@H]1[C@H]([C@H]([C@@H]([C@@H](O1)O[C@H]2[C@@H]([C@H](OC([C@@H]2NC(=O)C)O)CO)O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3)CO)O[C@H]4[C@H]([C@H]([C@@H]([C@H](O4)CO[C@@H]5[C@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O)O)O)O[C@H]6[C@@H]([C@H]([C@@H](CO6)O)O)O)O)NC(=O)C)O)O)O

2.2 Synonyms

2.2.1 MeSH Synonyms

1. Ananase

2. Bromelain

3. Bromelain Pos

4. Bromelain-pos

5. Bromelainpos

6. Bromelains

7. Bromelin

8. Bromelins

9. Dayto Anase

10. Debrase

11. Dontisanin

12. Extranase

13. Mucozym

14. Proteozym

15. Traumanase

2.2.2 Depositor-Supplied Synonyms

1. Muxf

2. M0xf(3)

3. Bromelain, Technical Grade

4. Chebi:53469

5. 9001-00-7

6. Fuc-alpha-(1->3)-[man-alpha-(1->6)-[xyl-beta-(1->2)]-man-beta-(1->4)-glcnac-beta-(1->4)]-glcnac

7. Alpha-l-fuc-(1->3)-[alpha-d-man-(1->6)-[beta-d-xyl-(1->2)]-beta-d-man-(1->4)-beta-d-glcnac-(1->4)]-d-glcnac

8. Alpha-l-fucopyranosyl-(1->3)-[alpha-d-mannopyranosyl-(1->6)-[beta-d-xylopyranosyl-(1->2)]-beta-d-mannopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-d-glucopyranosyl-(1->4)]-2-acetamido-2-deoxy-d-glucopyranose

9. Alpha-l-fucosyl-(1->3)-[alpha-d-mannosyl-(1->6)-[beta-d-xylosyl-(1->2)]-beta-d-mannosyl-(1->4)-n-acetyl-beta-d-glucosaminyl-(1->4)]-n-acetyl-d-glucosamine

10. Alpha-l-fucp-(1->3)-[alpha-d-manp-(1->6)-[beta-d-xylp-(1->2)]-beta-d-manp-(1->4)-beta-d-glcpnac-(1->4)]-d-glcpnac

11. Bromelaine

12. Epitope Id:115005

13. Q27124066

14. N_full_22100100000000_gs_1023_c1

15. Man(a1-6)[xyl(b1-2)]man(b1-4)glcnac(b1-4)[fuc(a1-3)]glcnac

16. Wurcs=2.0/6,6,5/[a2122h-1x_1-5_2*ncc/3=o][a1221m-1a_1-5][a2122h-1b_1-5_2*ncc/3=o][a1122h-1b_1-5][a212h-1b_1-5][a1122h-1a_1-5]/1-2-3-4-5-6/a3-b1_a4-c1_c4-d1_d2-e1_d6-f1

2.3 Create Date

2009-11-19

3 Chemical and Physical Properties

Molecular Formula	C₃₉H₆₆N₂O₂₉
Molecular Weight	1026.9 g/mol
XLogP3	-11.6
Hydrogen Bond Donor Count	18
Hydrogen Bond Acceptor Count	29
Rotatable Bond Count	16
Exact Mass	1026.37512407 g/mol
Monoisotopic Mass	1026.37512407 g/mol
Topological Polar Surface Area	483 Å²
Heavy Atom Count	70
Formal Charge	0
Complexity	1680
Isotope Atom Count	0
Defined Atom Stereocenter Count	28
Undefined Atom Stereocenter Count	1
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently Bonded Unit Count	1

4 Drug and Medication Information

4.1 Therapeutic Uses

Bromelain has /shown/ some success as a substitute for trypsin and pepsin in cases of pancreatic insufficiency and post-pancreatectomy.

Medical Economics Co; Physicians Desk Reference for Nutritional Supplements 1st ed p.72 (2001)

Acute postoperative and post-traumatic conditions of swelling, especially of the nasal and paranasal sinuses.

Blumenthal M, ed; The Complete German Commission E Monographs: Therapeutic Guide to Herbal Medicines p.94 (1998)

In an open case observation study involving patients with blunt injuries to the musculoskeletal system, the efficacy and tolerability of high-dose Bromelain POS, a plant-derived enzyme preparation, were investigated. The investigating physician was an orthopedic surgeon who, in addition to the usual therapeutic measures, treated 59 of his patients with the bromelaine preparation. The duration of the application was determined by the nature and severity of the lesion, and varied between one and three weeks. The test criteria were swelling, pain at rest and during movement, and tenderness. These parameters were evaluated on the day of the injury and on five subsequent dates. Treatment with bromelaine resulted in a clear reduction in all four parameters tested. Both swelling and the symptoms of pain had improved appreciably at all evaluation time points as compared with baseline. The tolerability of the preparation was very good, and patient compliance was correspondingly high.

PMID:7672747 Masson M; Fortschr Med 113 (19): 303-6 (1995)

4.2 Drug Warning

Gastrointestinal symptoms such as nausea and vomiting, diarrhea and cramping have been reported. There are occasional reports of metrorrhagia and menorrhagia.

Medical Economics Co; Physicians Desk Reference for Nutritional Supplements 1st ed p.72 (2001)

As with papain and other proteases, bromelain may cause contact allergenic reactions in certain individuals.

Leung, A.Y., Foster, S. Encyclopedia of Common Natural Ingredients Used in Food, Drugs, and Cosmetics. New York, NY. John Wiley & Sons, Inc. 1996., p. 101

Bromelain supplements should be avoided by pregnant women and nursing mothers.

Medical Economics Co; Physicians Desk Reference for Nutritional Supplements 1st ed p.70 (2001)

In one double-blind study of 73 patients with acute thrombophlebitis, bromelain, used with analgesics, reduced pain, edema, redness, tenderness, elevated skin temperature and disability.

Medical Economics Co; Physicians Desk Reference for Nutritional Supplements 1st ed p.72 (2001)

Those on anticoagulants or antithrombotic agents should exercise caution in the use of bromelain. Bromelain may have blood thinning activity in some.

Medical Economics Co; Physicians Desk Reference for Nutritional Supplements 1st ed p.70 (2001)

5 Pharmacology and Biochemistry

5.1 Absorption, Distribution and Excretion

Evidence that bromelains (I) is absorbed from the gastrointestinal tract of rats after oral administration is demonstrated in a bioavailability study in which 125I is given by oral route to rats and blood levels sampled at various times thereafter. A maximum level of 270 ng/ml I was found at one hr after administration.

PMID:3207859 White RR et al; Biopharm Drug Dispos 9 (Jul-Aug): 397-403 (1988)

Bromelain, a standardized complex of proteases from the pineapple plant, is absorbed unchanged from the intestine of animals at a rate of 40% ... .

PMID:2203073 Lotz-Winter H; Planta Med 56 (3): 249-53 (1990)

5.2 Mechanism of Action

It has been postulated that a minor enzymatic component present in bromelain is responsible for the release of a kinin, which stimulates the production of prostaglandin E1-like compounds; These PGE1-like compounds are then responsible for the physiological activities of bromelain.

Leung, A.Y., Foster, S. Encyclopedia of Common Natural Ingredients Used in Food, Drugs, and Cosmetics. New York, NY. John Wiley & Sons, Inc. 1996., p. 101

Bromelain's digestant activity is based on its ability to hydrolyze proteins to oligopeptides and amino acids. Bromelain's proteolytic enzymes are cysteine proteases. Cysteine proteases cleave peptide bonds by nucleophilic attack via active-site cysteine residues.

Medical Economics Co; Physicians Desk Reference for Nutritional Supplements 1st ed p.71 (2001)

The mechanism of the putative anti-inflammatory activity ... may be accounted for, in part, by activation of plasmin production from plasminogen and reduction of kinin via inhibition of the conversion of kininogen to kinin. Other possibilities, may include proteolytic degradation of circulating immune complexes and inhibition of signaling by extracellular regulated kinase (ERK-2) and p21 ras.

Medical Economics Co; Physicians Desk Reference for Nutritional Supplements 1st ed p.71 (2001)

Bromelain has been shown to increase CD2-mediated T cell activation, to enhance antigen-independent binding to monocytes and to increase interferon (IFN)-gamma-dependant, tumor necrosis factor (TNF)-alpha, Interleukin (IL)-1 beta, and interleukin (IL)-6 production in peripheral blood monocytes. These effects are thought to be due to bromelains proteolytic activity at cell surfaces, whereby it either removes surface molecules or reveals ones that already exist on cell membranes, thereby altering receptor-ligand interactions.

Medical Economics Co; Physicians Desk Reference for Nutritional Supplements 1st ed p.71 (2001)

For more Mechanism of Action (Complete) data for BROMELAINS (7 total), please visit the HSDB record page.

API SUPPLIERS

01

Zeon Pharma Industries India Pvt L...

India

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Jai Radhe Sales

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Apollo Ingredients India Pvt Ltd

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euroPLX 86 Munich

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Newgen Biotech USA

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Vaishali Pharma Ltd

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Guangxi Nanning Baihui Pharmaceuti...

China

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Beloorbayir Biotech

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Bio-gen Extracts

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Zeon Pharma Industries India Pvt Lt...

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About the Company : Zeon Pharma Industries India Pvt ltd is an ISO & GMP certified manufacturer of Bulk drugs & Intermediate and also supplies our associate manufacturing plant API / Semi finish form...

Zeon Pharma Industries India Pvt ltd is an ISO & GMP certified manufacturer of Bulk drugs & Intermediate and also supplies our associate manufacturing plant API / Semi finish formulations/Excipients, Phytochemicals/Enzymes & Intermediate and also has a GMP certified Manufacturing facility for Phytochemicals/Herbal extract and Enzymes through our associates.

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Jai Radhe Sales

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About the Company : Jai Radhe Sales was founded in 1999 as an out-of-the-box distribution firm specializing in the global supply of high-quality pharmaceutical ingredients. The firm provides complete ...

Jai Radhe Sales was founded in 1999 as an out-of-the-box distribution firm specializing in the global supply of high-quality pharmaceutical ingredients. The firm provides complete sourcing solutions for pharmaceutical products from India, including technical and regulatory assistance. It believes in providing its customers with high-quality products at reasonable prices. It has always endeavored to achieve global standards in the field of pharmaceuticals and has carved out a niche for itself through new methods based on current market needs. Today, it has established itself as a truly global company, with exports to almost every continent.

03

Beloorbayir Biotech

India

euroPLX 86 Munich

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04

Bio-gen Extracts

India

euroPLX 86 Munich

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Newgen Biotech USA

U.S.A

euroPLX 86 Munich

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Vaishali Pharma Ltd

India

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API Reference Price

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